Correction: A novel regulatory mechanism links PLCγ1 to PDK1.

Fig. 7. Simultaneous inhibition of PDK1 and PLCγ1 does not enhance the effect of inhibition of PDK1 or PLCγ1 alone on cell invasion. (A,B) Results from an invasion assay performed with MDA-MB-231 and A375M cells, respectively, expressing shRNA targeting PLCγ1 or a 3MUT non-targeting sequence, and treated with 1 μM GSK2334470. (C,D) Results from invasion assay performed with the indicated MDA-MB-231 and A375M stable cell populations transiently transfected with scrambled siRNA or siRNA targeting PLCγ1. In all panels data are the number of invaded cells per field expressed as a percentage of control (5MUT or 3MUT cells, respectively). Blots in figure show the levels of PDK1 or PLCγ1 in the indicated cells. Anti-ERK2 antibody was used as a loading control. Inserts show representative images stained with Crystal Violet. In all panels values are means ± s.e.m. from three independent experiments performed in duplicate. *P<0.05. **P<0.01.